Romero Garrido, Antonio
91 837 3112 Extension: 4244/4243
The research interests of the group are the structural characterization of target proteins and their complexes at atomic resolution using X-ray crystallography and other biophysical techniques. Specific research interests focus on transcription factors associated with tumorigenic processes and proteins involved in bacterial antibiotic resistance and bacterial pathogenesis. Proteins and pathways under study include periplasmic and cell wall proteins as well as more specialized secretion systems. The solved structures will provide the structural basis for unraveling the molecular mechanisms involved in pathogenesis at the atomic level.
Figure 1. Structural representation of OXA-24
Approaches to combat bacterial infection rely on 1) the disruption of the bacteria growth cycle by preventing the synthesis and assembly of key components of bacterial processes or 2) by inhibition of virulence traits. Our objective is to characterize those proteins involved in human infections at atomic resolution in order to understand the mechanisms of pathogenicity of several opportunistic human pathogens.
Figure 2. Triclosan induces antibiotic resistance in Stenotrophomonas maltophilia
For instance, an attractive approach to treat antibiotic-resistant microbial infections is to combine the β-lactam antibiotic with inhibitors of the microbial β-lactamase. However, although many ESBL-producing bacteria possess β-lactamases that are susceptible to β-lactamase inhibitors, the clinical efficacy of combined β-lactam/β-lactamase inhibitor still remains to be conclusively established. Besides, current commercial β-lactamase inhibitors (clavulanate, sulbactam and tazobactam) have narrow activity targeting only class A β-lactamases. Thus, there is an urgent need for the design and development of other β-lactamase inhibitors to widen the activity spectrum and generalize the action of combined treatments. It is foreseeable that out results will provide new leads to develop novel therapeutic approaches to combat bacterial infection.
Figure 3. Molecular architecture of assC, an Hcp-1 like protein from A. baumannii.
The group has extensive experience in structural biology, as well as in molecular biology and other structural biophysical techniques. The laboratory is fully equipped to carry out protein X-ray crystallography, including an in-house diffractometer, crystallization robots, and molecular biology-protein chemistry equipment.